The accuracy of mass spectrometry used to determine large molecular mass as proteins is often influenced by the isotopic compositions within a protein. Isotopic depletion is a powerful tool to resolve this problem. Using Fourier transform ion cyclotron resonance mass spectrometer, we investigated the ¹³C depleted cellular retinoic acid binding protein I, the measured mass accuracy is increased compared with the natural protein

KEY WORDS: Molecular mass, Mass accuracy, Isotopic depletion, Monoisotopic


Bull. Chem. Soc. Ethiop. 2009, 23(1), 101-103.