Biochemical genetic variation between four populations of Labeobarbus polylepis from three river systems in South Africa

  • PFS Mulder Department of Physiology, University of the North, Private Bag X1106, Sovenga 0727, South Africa
  • D Engelbrecht Department of Physiology, University of the North, Private Bag X1106, Sovenga 0727, South Africa
  • JS Engelbrecht Aquatic Research, Mpumalanga Parks Board, Private Bag X1088, Lydenburg 1120, South Africa
  • F Roux Aquatic Research, Mpumalanga Parks Board, Private Bag X1088, Lydenburg 1120, South Africa
Keywords: electrophoresis, Incomati River, Olifants River, Phongolo River

Abstract

The genetic variation within four Labeobarbus polylepis populations from both river and dam environments in the Limpopo, Incomati and Phongolo River systems was studied. Gene products of 22 enzyme-coding loci were resolved using horizontal starch gel electrophoresis. Fourteen (64%) of the 22 loci were monoallelic in all populations. Levels of polymorphism (P0.95) ranged between 9.1% and 22.7%. The heterozygosity varied from 0.028 for the Westoe Dam population (Phongolo River system) to 0.093 in the Spekboom River population (Limpopo River system). The genetic distance, FST and NEM values, as well as pair-wise contingency c2 analyses indicate a lack of gene flow between populations, as expected for isolated fish. Evidence of foreign genetic material in one population was also observed.

African Journal of Aquatic Science 2004, 29(1): 97–102
Published
2004-08-31
Section
Articles

Journal Identifiers


eISSN: 1727-9364
print ISSN: 1608-5914