Enzymatic activities as potential stress biomarkers of two substituted benzene compounds in Propsilocerus akamusi (Diptera: Chironomidae)

  • CW Cao
  • XP Li
  • SL Ge
  • LL Sun
  • ZY Wang

Abstract

Chironomids are a globally distributed family of insects that can serve as biological indicators of environmental pollution. Substituted benzenes are a group of serious environmental pollutants and severely threaten biological and human health. In order to investigate potential stress biomarkers of substituted benzenes, the effects of 4-chlorophenol and p-phenylene diamine on superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), carboxylesterase (CarE), acid phosphatase (ACP) and alkaline phosphatase (ALP) activities of 4th-instar larval Propsilocerus akamusi were investigated. During 48 h exposure to 4-chlorophenol and p-phenylene diamine, the larval CAT, SOD, GST and CarE activities responded to 4-chlorophenol and p-phenylene diamine in a concentrationand time-dependent way. The ACP and ALP activities of larval P. akamusi responded slowly to 4-chlorophenol and p-phenylene diamine, and only the ALP activities were inhibited significantly under the exposure to p-phenylene diamine for 6 and 24 h. These results suggest that CAT, SOD, GST and CarE activities of chironomids can be used as potential stress biomarkers to monitor 4-chlorophenol and p-phenylene diamine pollution.

Keywords: 4-chlorophenol, biochemical marker, p-phenylene diamine

African Journal of Aquatic Science 2012, 37(3): 265–270

Author Biographies

CW Cao
Department of Forest Protection, Northeast Forestry University, Harbin 150040, People’s Republic of China
XP Li
Department of Forest Protection, Northeast Forestry University, Harbin 150040, People’s Republic of China
SL Ge
Department of Forest Protection, Northeast Forestry University, Harbin 150040, People’s Republic of China
LL Sun
Department of Forest Protection, Northeast Forestry University, Harbin 150040, People’s Republic of China
ZY Wang
Department of Forest Protection, Northeast Forestry University, Harbin 150040, People’s Republic of China
Section
Articles

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eISSN: 1727-9364
print ISSN: 1608-5914