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Characterization of a newly isolated green microalga Scenedesmus sp. as a potential source of biodiesel


Juan Peng
Kai Yin
Jian-Ping Yuan
Guang-Xia Cao
Min Xue
Chou-Fei Wu
Jiang-Hai Wang

Abstract

An oil-producing microalga SP-01 belonging to the genus Scenedesmus was shown to be able to grow on a media containing 5 to 30 g L-1 NaCl. Under mixotrophic condition, the maximum cell dry weight of 3.1 g L-1 and specific growth rate of 0.034 h-1 were obtained at 0.5 g L-1 NaNO3 and 6 g L-1 acetate. The effects of salinity on the biomass, lipid, and carotenoid productions of the alga SP-01 in mixotrophic mode were investigated. The biomass productivity increased with increasing NaNO3 concentrations, and addition of NaCl resulted in a higher biomass while NaNO3 was present. The maximum lipid content was obtained while no NaNO3 and NaCl was added, and the lipid content decreased with increasing NaNO3 concentrations or addition of NaCl. The maximum lipid productivities of 67.44 to 68.44 mg L-1day-1 were obtained while NaNO3 and NaCl were not added or while 20 g L-1 NaCl and 0.13 g L-1 NaNO3 were added. The algal lipid was mainly composed of C16 and C18 fatty acids accounting for more than 90% of total fatty acids. Furthermore, lutein and astaxanthin were the main carotenoids.

Key words: Halotolerant, microalga, Scenedesmus sp., mixotrophic culture, lipid, carotenoid.


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eISSN: 1684-5315