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Possible oxidative effects of isotretinoin and modulatory effects of vitamins A and C in Saccharomyces cerevisiae


Alaíde Silva Lemos
Vanessa Leopoldino Coelho Rodrigues
Marcus Vinícius Oliveira Barros de Alencar
Md. Torequl Islam
Raí Pablo Sousa de Aguiar
Ana Maria Oliveira Ferreira da Mata
Ricardo Melo de Carvalho
Márcia Fernanda Correia Jardim Paz
Chistiane Mendes Feitosa
Ana Amélia de Carvalho Melo-Cavalcante

Abstract

Isotretinoin (ITN), chemically known as 13-cis-retinoic acid, is a part of the broad group of compounds related to vitamin A. It is particularly employed in the treatment of nodular cystic acne and as an inhibitor of proliferation of neoplastic cells, by exerting a regulatory effect on the cell differentiation. This study aimed at investigating the possible oxidative effects of ITN and modulatory effects of vitamins A and C in mutant and non-mutant Saccharomyces cerevisiae strains. In addition, to reconfirm the oxidative effects, five in vitro antioxidant assays were also prepared taking the alpha-tocopherol analogue, trolox as a standard. In vivo study conducted on S. cerevisiae cells was carried out with ITN 20 μg/ml taking hydrogen peroxide (H2O2) as stressor (STR), whereas ITN 5 to 50 μg/ml was considered for in vitro assays taking similar dose of trolox (TRO). Results show ITN to have oxidative effect in both in vitro and in vivo tests. In conclusion, ITN produced oxidative effects and there may be an hypervitaminosis effect with vitamins A and C, thus insinuation to genetic material.

Key words: Assay, isotretinoin, vitamin A, vitamin C, oxidative stress.


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eISSN: 1684-5315