Complete nucleotide sequencing, SNP identification and characterization of SRY gene in Indian Sangamneri goat

Abstract

The SRY gene is responsible for testis determination in mammals. It codes for a protein with a DNA binding domain similar to those found in high-mobility group protein (HMG box). Mutation in this gene leads to Gonadal Dysgenesis an abnormal sexual phenotype described in human, cattle, horse, and river buffalo. Here we report PCR-SSCP analysis and sequencing of coding region of SRY gene in Sangamneri goat (Capra hircus) using 40 male goats (bucks) samples. Polymorphism was detected in
only one sample indicating that a major portion of SRY gene is highly conserved in this breed.