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African Journal of Biotechnology

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Optimized integration of T-DNA in the taxol-producing fungus Pestalotiopsis malicola

X Hao, Y Ji, S Liu, J Bi, Q Bi, J Pan, X Zhu

Abstract


We previously reported a taxol-producing fungus Pestalotiopsis malicola. There, we described the transformation of the fungus mediated by Agrobacterium tumefaciens. T-DNA carrying the selection marker was transferred into the fungus and randomly integrated into the genome as shown by Southern blotting. Approximately 66% of the transformants obtained a single copy of T-DNA. Conditions of transformation were optimized. The method can be used for taxol-related genes searching and manipulation in P. malicola. This is the first report of T-DNA-mediated transformation in taxol-producing fungi.

Key words: T-DNA, random insertional mutagenesis, taxol, Pestalotiopsis.




http://dx.doi.org/10.5897/AJB11.2715
AJOL African Journals Online