Optimized integration of T-DNA in the taxol-producing fungus Pestalotiopsis malicola

  • X Hao
  • Y Ji
  • S Liu
  • J Bi
  • Q Bi
  • J Pan
  • X Zhu
Keywords: T-DNA, random insertional mutagenesis, taxol, Pestalotiopsis.

Abstract

We previously reported a taxol-producing fungus Pestalotiopsis malicola. There, we described the transformation of the fungus mediated by Agrobacterium tumefaciens. T-DNA carrying the selection marker was transferred into the fungus and randomly integrated into the genome as shown by Southern blotting. Approximately 66% of the transformants obtained a single copy of T-DNA. Conditions of transformation were optimized. The method can be used for taxol-related genes searching and manipulation in P. malicola. This is the first report of T-DNA-mediated transformation in taxol-producing fungi.

Key words: T-DNA, random insertional mutagenesis, taxol, Pestalotiopsis.

Published
2014-01-28
Section
Articles

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eISSN: 1684-5315