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African Journal of Biotechnology

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Isolation and partial purification of lysozyme from saliva of Bali cattle (Bos sondaicus) using an aqueous mixture of polyethylene glycol (PEG) with sodium sulfate

SN Depamede, N Asri, NI Julisaniah, BF Suryadi, D Kisworo

Abstract


Lysozyme from saliva of Bali cattle (Bos sondaicus) was successfully isolated through an aqueous mixture system of polyethylene glycol (PEG 4000) and salt (Na2SO4). About 100 μg/ml lysozyme of 14.2 kDa, determined through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), per 4800 μg/ml saliva was obtained. Based on turbidity assay of Staphylococcus aureus, it was revealed that inhibition activities of 40 μg/ml isolated lysozyme were comparable to 800 μg/ml of a commercial hen egg white lysozyme.

Key words: Lysozyme, Bali cattle, Bos sondaicus, PEG, aqueous mixture system.




http://dx.doi.org/10.5897/AJB11.2529
AJOL African Journals Online