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African Journal of Biotechnology

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Genetic diversity among four Eucalyptus species (myrtaceae) based on random amplified polymorphic DNA (RAPD) analysis

AK Osman, AA El-Mageed, AQ Tawfik, HA Mohammed

Abstract


Results obtained from random amplified polymorphic DNA (RAPD) analysis of four species of the genus Eucalyptus revealed that the number of fragments amplified for each of the five studied primer varied between 13 and 14  fragments and their size ranged from 95 to 1055 bp. The total number of
bands was 67, total number of monomorphic bands was 32, total number of polymorphic bands was 35, and the polymorphism percentage was 52.2%. The results indicate that 16 positive and 13 negative markers were detected. The marker fragments size ranged between 175 to 630 bp for the negative
markers and 235 to 945 bp for the positive markers.

Key words: Eucalyptus, random amplified polymorphic DNA (RAPD), genetic diversity, DNA fingerprinting PCR, dendrogram




http://dx.doi.org/10.5897/AJB11.3239
AJOL African Journals Online