Assessment of genetic relatedness of the two Amaranthus retroflexus populations by protein and random amplified polymorphic DNA (RAPD) markers

  • Drinic Mladenovic Snezana
  • Kostadinovic Marija
  • Ristic Danijela
  • Simic Milena
  • Stefanovic Lidija

Abstract

Two populations of Amaranthus retroflexus with different morphology were collected from field of the Maize Research Institute Zemun Polje, Serbia. Random amplified polymorphic DNA (RAPD) and seed protein analysis were performed to study the genetic differences in two grain Amaranthus populations. The studied populations have different protein and DNA profile. A total of 171 DNA fragments were generated by 31 RAPD primers, with an average of 5.5 fragments per primer. Of these, 61.4% fragments were polymorphic among the two populations. 18 protein fraction were obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). The populations differed in the four protein fractions of different molecular weight. The seed protein electrophoresis and RAPD markers are useful for genetic determination of A. retroflexus populations and identification of biotypes with atypical morphology.

Keywords: Amaranthus retroflexus, biotypes, molecular markers, proteins.

African Journal of Biotechnology Vol. 11(29), pp. 7331-7337, 10 April, 2012

Author Biographies

Drinic Mladenovic Snezana
Maize Research Institute Zemun Polje, Belgrade, Serbia
Kostadinovic Marija
Maize Research Institute Zemun Polje, Belgrade, Serbia
Ristic Danijela
Maize Research Institute Zemun Polje, Belgrade, Serbia
Simic Milena
Maize Research Institute Zemun Polje, Belgrade, Serbia
Stefanovic Lidija
Maize Research Institute Zemun Polje, Belgrade, Serbia
Published
2014-04-02
Section
Articles

Journal Identifiers


eISSN: 1684-5315