Polymorphism of the prolactin gene (PRL) and its relationship with milk production in American Swiss cattle

  • Edy Alfonso
  • Reyna Rojas
  • José G Herrera
  • María E Ortega
  • Clemente Lemus
  • César Cortez
  • Jaime Ruiz
  • René Pinto
  • Heriberto Gómez

Abstract

The modern dairy cattle breeding strategy in the Mexican tropic is to identify genes or allelic variants that can be incorporated into selection programs such as the prolactin gene (PRL) which is associated with milk production and quality. The aim of this study is to screen an American Swiss population in Chiapas, Mexico, in order to analyze the polymorphism of the prolactin gene as well as its relationship with milk production in blood samples of 417 American Swiss cattle. The genotypes were determined through the polymerase chain reaction-restriction fragments length polymorphism (PCR-RFLP) technique, using RsaI restriction endonuclease, showing a 156 bp fragment located in exon 3. Allele frequencies in the studied breed were: A = 0.8765 and B = 0.1235. The genotype frequencies of AA, AB and BB were 0.776, 0.174 and 0.026, respectively. The Chi-square indicated that genotype distributions were not in the Hardy-Weinberg equilibrium (P<0.05). The results show that animals with genotype AA had a greater milk production during lactation than genotypes AB and BB (P<0.05), with genotype BB being the one that had the lowest production (P<0.05). It was concluded that the identification of the prolactin polymorphism in this population will allow the achievement of a better efficiency in the selection of breeding animals.

Keywords: Brown Swiss, prolactin, polymorphism, milk, RFLP-RsaI.

African Journal of Biotechnology Vol. 11(29), pp. 7338-7343, 10 April, 2012

Author Biographies

Edy Alfonso
Colegio de Posgraduados. Recursos Genéticos y Productividad-Ganadería. Carretera México-Texcoco km 36.5. Montecillo, Estado de México. CP 56230
Reyna Rojas
Colegio de Posgraduados. Recursos Genéticos y Productividad-Ganadería. Carretera México-Texcoco km 36.5. Montecillo, Estado de México. CP 56230
José G Herrera
Colegio de Posgraduados. Recursos Genéticos y Productividad-Ganadería. Carretera México-Texcoco km 36.5. Montecillo, Estado de México. CP 56230
María E Ortega
Colegio de Posgraduados. Recursos Genéticos y Productividad-Ganadería. Carretera México-Texcoco km 36.5. Montecillo, Estado de México. CP 56230
Clemente Lemus
Unidad Acadèmica de Medicina Veterinaria y Zootecnia, Posgrado CBAP-UAN. Tepic, Nayarit, México
César Cortez
Colegio de Posgraduados. Recursos Genéticos y Productividad-Ganadería. Carretera México-Texcoco km 36.5. Montecillo, Estado de México. CP 56230
Jaime Ruiz
Facultad de Medicina Veterinaria y Zootecnia, UNACH. Tuxtla Gutiérrez, Chiapas, México
René Pinto
Facultad de Ciencias Agronómicas. UNACH. Villaflores, Chiapas, México
Heriberto Gómez
Facultad de Ciencias Agronómicas. UNACH. Villaflores, Chiapas, México
Published
2014-04-02
Section
Articles

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