Genetic evaluation of domestic walnut cultivars trading on Korean tree markets using microsatellite markers

  • Ho Bang Kim
  • Ji Hyun Jeon
  • A Reum Han
  • Yi Lee
  • Sung-Soo Jun
  • Tae-Houn Kim
  • Gun-Hyoung Cho
  • Phun Bum Park

Abstract

Walnut (Juglans regia L.) is regarded as a healthy food because of its high nutritional composition and various health benefits. Although several walnut cultivars are being actively traded for domestic plantation or ornament in Korea, no particular effort has been made to evaluate genetic quality management of walnut cultivars in domestic tree markets. In this study, as an effort to evaluate the status of walnut seedling trade, we collected walnut seedlings of diverse cultivars from domestic tree markets and several locations in Korea and performed genotype analysis for the collections using microsatellite markers (76 individuals belonging to eight domestic cultivars). We used 12 markers that were previously reported to be informative and polymorphic in walnuts. The number of alleles that was detected from the collections using these markers ranged from 6 to 16, with heterozygosity values ranging from 0.03 to 0.75. Dendrogram revealed that the domestic walnut cultivars trading on tree markets could be genotypically distinguished from various foreign cultivars. However, genotyping data also showed that individual plants belonging to identical cultivars were sporadically distributed on the dendrogram, indicating that walnut cultivars trading on domestic tree markets seem to be poorly managed.

Keywords: Walnut (Juglans regia L.), microsatellite markers, tree market, seedling trade, domestic cultivars, foreign cultivars, cultivar management

African Journal of Biotechnology Vol. 11(29), pp. 7366-7374, 10 April, 2012

Author Biographies

Ho Bang Kim
Life Sciences Research Institute, Biomedic Co., Ltd., Bucheon 420-852, Republic of Korea
Ji Hyun Jeon
Life Sciences Research Institute, Biomedic Co., Ltd., Bucheon 420-852, Republic of Korea
A Reum Han
Life Sciences Research Institute, Biomedic Co., Ltd., Bucheon 420-852, Republic of Korea
Yi Lee
Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju 361-763, Republic of Korea
Sung-Soo Jun
School of General Education, Institute for Scientifically Able Youth, Kyungwon University, Seoungnam 461-701, Republic of Korea
Tae-Houn Kim
Department of PrePharmMed, College of Natural Sciences, Duksung Women's University, Seoul 132-714, Republic of Korea
Gun-Hyoung Cho
Life Sciences Research Institute, Biomedic Co., Ltd., Bucheon 420-852, Republic of Korea
Phun Bum Park
Department of Bioscience and Biotechnology, University of Suwon, Hwaseong 445-743, Republic of Korea
Published
2014-04-02
Section
Articles

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eISSN: 1684-5315