Effects of different media concentartions on callogensis in sugar cane (Saccharum officinarum L.)

  • Waleed Ahmad Khattak
  • Mazhar Ul-Islam
  • Ihsan Ullah

Abstract

Sugarcane (Saccharum officinarum L) var. 668 was propagated through tissue culture technique: callogenesis at MS media supplemented with different concentrations of growth regulators. Explants were disinfected with 70% ethanol which was sprayed directly on outer most sheathe which covered the tender stem to be used as source of explants. After 15 days, those explants which survived turned into green leaves and were used for the aforementioned study. Different concentrations of growth regulators were applied. For callogensis response from the cultivated explant, combination of Murashige and Skoog (MS) + 2,4-dichlorophenoxy acetic acid (2,4.D) + benzylaminopurine (BAP) with different concentration was applied and were found best for the establishment of callus from shoot culture.

Keywords: Saccharum officinarum L, callogenesis, growth regulators, explants, shoot culture

African Journal of Biotechnology, Vol 13(11), 1219-1222

Author Biographies

Waleed Ahmad Khattak
Sarhad University of Science and Information Technology, Peshawar, Pakistan
Mazhar Ul-Islam
Department of Chemical Engineering, Daegu, South Korea
Ihsan Ullah
Sarhad University of Science and Information Technology, Peshawar, Pakistan; Institute of Biotechnology and Genetic Engineering KPK Agricultural University Peshawar, Pakistan
Published
2015-06-03
Section
Articles

Journal Identifiers


eISSN: 1684-5315