In vitro microrhizome production in Decalepis hamiltonii

  • K Thangavel
  • P Ravichandran
  • MG Ebbie
  • V Manimekalai

Abstract

Microrhizomes were produced from leaf disc derived callus of a threatened endemic medicinal plant - Decalepis hamiltonii. Murashige and Skoog (MS) medium supplemented with 2 mM 6-benzyleaminopurine (BAP) and 6 mM 1-napthaleneacetic (NAA) acid was found to be optimum for rapid callus induction and establishment from leaf disc explants. Further differentiation of callus into microrhizome was conquered in MS medium supplemented with 4 mM indole-3-butyric acid (IBA) and 8 mM NAA. A maximum of 20 microrhizomes in a cluster was produced within 90 days. Yeast extract (0.05%) and polyvinylpyrrolidone (0.05%) further enhanced the microrhizome formation when supplemented along with plant growth regulators (PGRs).

Keywords: Decalepis hamiltonii, microrhizome, herbal medicine, secondary metabolites

African Journal of Biotechnology, Vol 13(11), 1308-1313

Author Biographies

K Thangavel
School of Biological Sciences, Madurai Kamaraj University, Madurai - 625 021, India
P Ravichandran
Plant Biotechnology Laboratory, Sri Paramakalyani Centre for Environmental Sciences Manonmaniam Sudaranar University, Alwarkurichi - 627 412, India
MG Ebbie
Plant Biotechnology Laboratory, Sri Paramakalyani Centre for Environmental Sciences Manonmaniam Sudaranar University, Alwarkurichi - 627 412, India
V Manimekalai
Department of Botany, Sri Parasakthi College for Women (Autonomous), Courtallam - 627 802, India
Published
2015-06-10
Section
Articles

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eISSN: 1684-5315