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African Journal of Biotechnology

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Sequence analysis of mitochondrial DNA hypervariable region III of 400 Iraqi volunteers

HA Shneewer, NG Al-Loza, NA Kareem, IH Hameed

Abstract


The aims of this research were to study mitochondrial DNA hypervariable region III and establish the degree of variation characteristic of a fragment. The mitochondrial DNA (mtDNA) is a small circular genome located within the mitochondria in the cytoplasm of the cell and a smaller 1.2 kb pair fragment, called the control region (D-loop). DNA was extracted from blood following a standard phenol-chloroform method. Polymerase chain reaction (PCR) products were purified by EZ-10 spin column then sequenced and detected by using the ABI 3130xL DNA analyzer. New polymorphic positions detected as T460A, C426G, T471A, T482A, A493T and C518G may in future be suitable sources for identification purpose.

Keywords: Frequency, HVIII, mitochondrial DNA, polymorphic positions.




AJOL African Journals Online