Evaluation of genotoxicity of profenofos to freshwater fish Channa punctatus (Bloch) using the micronucleus assay

  • AK Pandey
  • NS Nagpure
  • SP Trivedi

Abstract

The present study investigates the genotoxicity of profenofos (PFF), an organophosphate pesticide in erythrocyte cells of freshwater fish, Channa punctata (Bloch) using micronucleus assay. The 96 h LC50 value of PFF (50% EC) was estimated for the fish species in a semistatic system and then 50% of LC50 was determined as 1.15 ppb. The fish specimens were exposed to 1.15 ppb sublethal concentration of the pesticide and samplings were done on 24, 48, 72 and 96 h post exposure for assessment of DNA damage by micronucleus assay. The study confirms that PFF is toxic for aquatic organism and the micronucleus assay is a useful tool in determining the potential genotoxicity and mutagenicity of xenobiotic compounds and can be considered as sensitive parameter for toxicity monitoring program.

Keywords: Genotoxicity, profenofos, Channa punctatus, DNA damage, micronucleus assay.

African Journal of Biotechnology, Vol 13(39) 3985-3988

Author Biographies

AK Pandey
Molecular Biology and Biotechnology Division, National Bureau of Fish Genetic Resources, Lucknow-226002, UP, India
NS Nagpure
Molecular Biology and Biotechnology Division, National Bureau of Fish Genetic Resources, Lucknow-226002, UP, India
SP Trivedi
Environmental Toxicology Laboratory, Department of Zoology, University of Lucknow, Lucknow, UP, India
Published
2015-10-12
Section
Articles

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eISSN: 1684-5315