Screening of thermophilic neutral lipase-producing Pseudomonas sp. ZBC1 and improving its enzymatic activity

  • Cheng Xing
  • Pu You-guang
  • Zhu Chen-xi
  • Ren Zhi-lin
  • Jiang Ming-xing
  • Chen Yan
  • Zhang Bu-chang

Abstract

From oil-contaminated soil, three lipase-producing microorganisms were selected as good lipase producers using rhodamine B-olive oil plate agar and they were identified as from Pseudomonas, Burkholderia and Klebsiella genera by morphology, biochemical characterization and 16S rRNA gene sequencing. Among the three strains, Pseudomonas sp. ZBC1 was found to produce thermophilic neutral lipase which exhibited maximum activity at 80ºC and pH 7.0. Plackett-Burman design (PB design) and response surface methodology (RSM) analysis showed that the optimum values of the most significant factors are MgSO4 (0.85 g/L), inoculum size (65.9 ml/L) and volume of medium (51.57 ml). Under the optimal conditions, lipase production by Pseudomonas sp. ZBC1 was enhanced by approximately 2.30 fold (from 8.50 to 19.50 U/ml).

Keywords: Pseudomonas, lipase, thermophilic, response surface methodology

African Journal of Biotechnology Vol. 12(9), pp. 949-957

Author Biographies

Cheng Xing
Institute of Health Sciences, Anhui University, Hefei, Anhui 230601, China; Kangermei Biotechnology R and D Center, Anhui University, Hefei, Anhui 230601, China; School of Life Sciences, Anhui University, Hefei, Anhui 230601, China
Pu You-guang
Institute of Health Sciences, Anhui University, Hefei, Anhui 230601, China; Kangermei Biotechnology R and D Center, Anhui University, Hefei, Anhui 230601, China; School of Life Sciences, Anhui University, Hefei, Anhui 230601, China
Zhu Chen-xi
Institute of Health Sciences, Anhui University, Hefei, Anhui 230601, China; Kangermei Biotechnology R and D Center, Anhui University, Hefei, Anhui 230601, China; School of Life Sciences, Anhui University, Hefei, Anhui 230601, China
Ren Zhi-lin
Kangermei Biotechnology R and D Center, Anhui University, Hefei, Anhui 230601, China
Jiang Ming-xing
Kangermei Biotechnology R and D Center, Anhui University, Hefei, Anhui 230601, China
Chen Yan
Institute of Health Sciences, Anhui University, Hefei, Anhui 230601, China; Kangermei Biotechnology R and D Center, Anhui University, Hefei, Anhui 230601, China; School of Life Sciences, Anhui University, Hefei, Anhui 230601, China
Zhang Bu-chang
Institute of Health Sciences, Anhui University, Hefei, Anhui 230601, China; Kangermei Biotechnology R and D Center, Anhui University, Hefei, Anhui 230601, China; School of Life Sciences, Anhui University, Hefei, Anhui 230601, China
Published
2015-12-05
Section
Articles

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eISSN: 1684-5315