The sequence-related amplified polymorphism (SRAP) markers linked to the color around the stone (Cs) locus of peach fruit
The distribution and concentration of anthocyanin, a major subgroup of the flavonoids and important determinants of peach fruit flesh color, is regulated by several genes. In the international reference Prunus map, the locus of flesh color around the stone (Cs) is located on linkage group LG3. This report describes the development of a sequence-related amplified polymorphism (SRAP) marker linked to major genes controlling the Cs in peach. One F1 progeny from a cross between the peach cultivars ‘Chongyanghong’ × ‘Yanhong’ segregated by fruit flesh color around the stone was used for bulked segregant analysis. Two kinds of bulked DNA were constructed and used for the polymerase chain reaction in SRAP analysis. After 88 pairs of primers were screened against the two kinds of bulked DNA, three dominant SRAP markers were selected in cooperation with the Cs bulk. One of the three markers (Me07Em02) was linked to the Cs locus at a distance of 0 cM. The SRAP markers would provide an effective tool for marker-assisted selection for the trait of anthocyanin intensity of peach flesh in peach breeding programs.
Key words: Prunus persica (L.) Batsch, flesh color around the stone, sequence-related amplified polymorphism (SRAP).