Main Article Content
Extracts of two spices namely ginger (Zingiber officinale) and black pepper (Piper guinenses) were prepared in 0.4, 1.2, 2.4 and 3.6% concentrations. Soymilk and kunuzaki, were treated, respectively with the different concentrations and stored at ambient temperature for 5 days. The microbial load and identification were determined every day of storage until samples were adjudged spoiled. On the first day, 0.4% ginger extract in soymilk and kunuzaki had a microbial load of 7.77 × 106b and 5.17 × 106b, respectively. 3.6% ginger extract in soymilk and kunzakki recorded 3.73 × 106b and 3.30 × 106 each. 0.4% black pepper extract in soymilk had 6.273 × 106b and recorded 4.63 × 106b in kunuzaki. 3.6% black pepper extract in soymilk and kunuzaki had a microbial load of 3.20 × 106d and 2.90 × 106c, respectively. On the 3rd day, the microbial load increased for both ginger and black pepper extract. Ginger extract recorded 9.13 × 106b in soymilk and 5.60 × 106b in kunuzaki at 0.4% concentration. Black pepper extracts recorded 7.43 × 106b in soymilk and 3.27 × 106b in kunuzaki also at 0.4% extract. 3.6% black pepper extract recorded 4.10 × 106a in soymilk and 2.20 × 106c in kunuzaki. There was linear reduction of microbial load as spice concentration increases. Black pepper recorded lower microbial load, thus has more anti microbial activity and may be preferred to be used as national anti microbial preservatives to extend the shelf-life of food.
Keywords: Zingiber officinale, Piper guinenses, soymilk, kunzakki