Effect of 6-benzyl aminopurine (BAP) on meristem culture for virus free seed production of some popular potato varieties in Bangladesh

  • Md. Afzal Hossain
  • Khondoker M Nasiruddin
  • Md. Abu Kawochar

Abstract

The present study was undertaken to fix the suitable concentration of 6-benzyl aminopurine on shoot development from meristem for producing virus free potato plantlet. The experiment consisted of five potato cultivars namely Diamant, Heera, Dheera, Granula and Cardinal for meristem culture and four 6-benzyl aminopurine levels namely 0, 1.0, 1.5, 2.0 mg/l. As a whole, twenty treatments were allotted in complete randomized design with three replications. Resulted in vitro regenerated plantlets were used as treatment for double antibody sandwich (DAS-), enzyme linked immunosorbent assay (ELISA) test, while, the infected plant parts were used as positive control. In virus elimination through meristem culture, Cardinal with 1 mg l-1 6-benzyl aminopurine gave maximum number of shoots (2.43 cm) explant-1, whereas, Dheera with 1.5 mg l-1 6-benzyl aminopurine gave the tallest plantlet (5.23 cm). On the other hand, explants of Dheera and Cardinal with 1.5 mg l-1 6-benzyl aminopurine and explants of Diamant, Heera, Granula and Cardinal with 2.0 mg l-1 6-benzyl aminopurine produced no roots. Finally, after DAS-ELISA test, the infected field samples developed yellow color but in vitro regenerated plantlets of all the varieties under study showed 100% virus freeness.

Keywords: Virus elimination, meristem culture, virus detection, seed potato

African Journal of Biotechnology Vol. 12(18), pp. 2406-2413

Author Biographies

Md. Afzal Hossain
Internal Resources Division, Ministry of Finance, Bangladesh Secretariat, Bangladesh.
Khondoker M Nasiruddin
Department of Biotechnology, Bangladesh Agricultural University, Bangladesh
Md. Abu Kawochar
Tuber Crops Research Center, Bangladesh Agricultural Research Institute, Bangladesh
Published
2016-01-30
Section
Articles

Journal Identifiers


eISSN: 1684-5315