Co-inoculation effects of Bradyrhizobium japonicum and Azospirillum sp. on competitive nodulation and rhizosphere eubacterial community structures of soybean under rhizobia-established soil conditions

  • Thi Thi Aung
  • Panlada Tittabutr
  • Nantakorn Boonkerd
  • David Herridge
  • Neung Teaumroong

Abstract

Bradyrhizobial inoculants used for soybean seed inoculation to maximize the benefit of N2-fixation should include bradyrhizobial strain with high N2-fixation rates and ability to compete with the indigenous rhizobial populations. In this study, co-inoculation of plant growth promoting rhizobacteria (PGPR) Azospirillum sp. with either of Bradyrhizobium japonicum CB 1809 or USDA 110 increased shoot and root dry weight of soybean over non-inoculated control under pot condition with no indigenous soybean nodulating bradyrhizobia. Moreover, competition for nodulation and the effects on rhizosphere soil eubacterial community structures by using single or co-inoculation of B. japonicum and Azospirillum sp. under rhizobia-established Myanmar and Thailand soils were investigated. By inoculation of gus-marked USDA 110 singly or its co-inoculation gave 93.21 to 94.75% and 74.21 to 100% in nodule occupancy, and 23.50 to 41.95% and 50.37 to 73.24% promotion in biomass dry weight over non-inoculated control in Myanmar and Thailand soil samples, respectively. Each of all the tested inoculum levels, that is 106, 107 and 108 cfu/ml of Azospirillum sp. enhanced nodulation in combination with USDA 110 with a corresponding increase in 73.8, 62.25 and 95.34%; and 51.52, 62.38 and 79.46% over non-inoculated control, respectively in Myanmar and Thailand soil, respectively. In addition, soybean rhizosphere soil eubacterial community structures were not shifted by bacterial inoculation. Therefore, Azospirillum sp. could be used in co-inoculant production with B. japonicum for soybean.

Keywords: Bradyrhizobium, plant growth promoting rhizobacteria (PGPR), soybean, co-inoculation, competition, rhizosphere eubacterial community structure

African Journal of Biotechnology Vol. 12(20), pp. 2850-2862

Author Biographies

Thi Thi Aung
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand
Panlada Tittabutr
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand
Nantakorn Boonkerd
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand
David Herridge
Primary Industries Innovation Centre (PIIC), University of New England, Australia
Neung Teaumroong
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand
Published
2016-03-01
Section
Articles

Journal Identifiers


eISSN: 1684-5315