Volatile metabolites profiling of a Chinese mangrove endophytic Pestalotiopsis sp. strain

  • Donghai Li
  • Shihong Yan
  • Peter Proksch
  • Zhenyi Liang
  • Qiang Li
  • Jing Xu

Abstract

Pestalotiopsis JCM2A4, an endophytic fungus originally isolated from leaves of the Chinese mangrove plant Rhizophora mucronata, produces a mixture of volatile metabolites. As determined by gas chromatography and gas chromatography/mass spectrometry (GC/GC-MS), 18 compounds representing all of the hexane extract were identified. Higher amounts of oil-based straight-chained alkyl (mono- and di-methyl) esters and fatty acids were found to compose major volatile chemotype which accounted for 78.65 and 14.52% of this organism, respectively. The main components was demonstrated to be pentadecanoic acid, 14-methyl-, methyl ester (35.92%); octadecanoic acid, methyl ester (13.10%); nonanedioic acid, dimethyl ester (11.21%); and n-hexadecanoic acid (10.54%). Two of these components were isolated and determined to be n-hexadecanoic acid and elaidic acid by 1H NMR and 1H-1H COSY spectroscopy. Antioxidant activity of the hexane extract and isolated compounds were screened using 2,2'-diphenyl-b-picrylhydrazyl (DPPH) free radical scavenging method. This is the first report to describe the volatile metabolites of mangrove endophytic Pestalotiopsis sp. strain; its specific fatty acid methyl esters (FAME) profile can be used as a tool for microbial source tracking.

Keywords: Mangrove endophytic fungus, Pestalotiopsis sp., volatile metabolites, fatty acid methyl esters (FAME) profile

African Journal of Biotechnology Vol. 12(24), pp. 3802-3806

Author Biographies

Donghai Li
Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources, Ministry of Education, College of Material and Chemical Engineering, Hainan University, Haikou 570228, People’s Republic of China
Shihong Yan
Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources, Ministry of Education, College of Material and Chemical Engineering, Hainan University, Haikou 570228, People’s Republic of China
Peter Proksch
Institut für Pharmazeutische Biologie und Biotechnologie, Heinrich-Heine-Universität, Geb. 26.23, Universitätsstrasse 1, D-40225 Düsseldorf, Germany
Zhenyi Liang
Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources, Ministry of Education, College of Material and Chemical Engineering, Hainan University, Haikou 570228, People’s Republic of China
Qiang Li
key Laboratory Of Water Pollution Treatment and resource reuse of Hainan province, Hainan Normal University, Haikou 571158 People’s Republic of China
Jing Xu
Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources, Ministry of Education, College of Material and Chemical Engineering, Hainan University, Haikou 570228, People’s Republic of China; Key Laboratory Of Water Pollution Treatment and resource reuse of Hainan province, Hainan Normal University, Haikou 571158 People’s Republic of China; Institut für Pharmazeutische Biologie und Biotechnologie, Heinrich-Heine-Universität, Geb. 26.23, Universitätsstrasse 1, D-40225 Düsseldorf, Germany
Published
2016-03-16
Section
Articles

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eISSN: 1684-5315