Molecular investigations on grain filling rate under terminal heat stress in bread wheat (Triticum aestivum L.)

  • Girish Chandra Pandey
  • Jagadish Rane
  • Sindhu Sareen
  • Priyanka Siwach
  • NK Singh
  • Ratan Tiwari


Grain yield under post anthesis high temperature stress is largely influenced by grain filling rate (GFR). To investigate molecular basis of this trait, a set of 111 recombinant inbred lines (RILs) derived from Raj 4014, a heat sensitive genotype and WH 730, heat tolerant cultivar was phenotyped during 2009-2010 and 2010-2011 crop seasons, under field conditions. The difference in GFR (dGFR) between the timely and late sown conditions was used as a phenotypic parameter to find association with molecular markers, as parental lines exhibited significant difference for this trait. The mapping population showed clear-cut segregation pattern for differences in GFR between timely and late sown conditions. About 75% of the progenies showed no difference while 25% showed significant difference in GFR under high temperature stress created by late sown condition. To study the association of this trait with the markers, the parental lines were screened with 300 simple sequence repeat (SSR) microsatellite markers out of which 15% (45) were polymorphic between parental lines. These polymorphic markers were utilized for genotyping a subset, comprising of 43 RILs that had clear contrasting variation for dGFR. Regression analysis revealed significant association of dGFR of RILs with two markers viz., Xbarc04 and Xgwm314 with coefficients of determination (R2) values of 0.10 and 0.06, respectively.

Keywords: Grain filling rate (GFR), simple sequence repeat (SSR), heat tolerance, wheat

African Journal of Biotechnology Vol. 12(28), pp. 4439-4445

Author Biographies

Girish Chandra Pandey
Directorate of Wheat Research, Karnal-132001, Haryana, India
Jagadish Rane
National Institute of Abiotic Stress Management, Malegaon, Baramati, Pune-413115, India.
Sindhu Sareen
Directorate of Wheat Research, Karnal-132001, Haryana, India
Priyanka Siwach
Ch. Devi Lal University, Sirsa-125055, Haryana, India.
NK Singh
National Research Center on Plant Biotechnology, New Delhi-110012, India.
Ratan Tiwari
Directorate of Wheat Research, Karnal-132001, Haryana, India.

Journal Identifiers

eISSN: 1684-5315