Regeneration of plantlets from unpollinated ovary cultures of Ethiopian wheat (Triticum turgidum and Triticum aestivum)

  • Tsegaye Getahun
  • Tileye Feyissa
  • Likyelesh Gugsa

Abstract

An in vitro culture protocol was established for direct regeneration of plantlets from unpollinated ovary cultures of four Ethiopian wheat varieties. Unpollinated ovaries were excised from durum wheat (Yerer and Ude varieties) and bread wheat (Simba and Galama varieties). Analysis of variance (ANOVA) has shown that genotypes, types of media, concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D) and kinetin (KIN) and durations of cold pretreatment at 4°C significantly (P≤0.05) affected direct formation of embryonic tissues independently. Stage II of wheat spikes, MS medium containing 1 mg/l of each of 2,4-D and KIN and 15 days of cold pretreatment were found to be the best conditions for direct formation of embryonic tissues. The highest frequency of shoots were regenerated from the cultured embryonic tissues of Yerer (41.6% ) and Simba (41.3%) on medium containing 0.1 mg/l 2,4-D. From a total of 14,524 cultured unpollinated ovaries, 1,100 embryonic tissues (7.6 %) and 75 regenerants were obtained. The average percentage of embryonic tissues and regenerants were 9.0 and 1.1% from 3,444; 9.8 and 0.55% from 4,732; 5.6 and 0.17% from 2,988; 4.7 and 0.12% from 3,360 cultured unpollinated ovaries for varieties Yerer, Simba, Ude and Galama, respectively.

Keywords: Embryonic tissues, Unpollinated ovaries, regenerants, wheat varieties

African Journal of Biotechnology Vol. 12(39), pp. 5754-5760

Author Biographies

Tsegaye Getahun
Department of Biology, University of Gondar, P.O. Box 196, Gondar, Ethiopia; Microbial,Cellular and Molecular Biology Program Unit, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia
Tileye Feyissa
Microbial,Cellular and Molecular Biology Program Unit, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia
Likyelesh Gugsa
Ethiopian Institute of Agricultural Research (EIAR): Holetta Agricultural Research Center (HARC), P.O. Box 31, Holetta, Ethiopia
Published
2016-05-16
Section
Articles

Journal Identifiers


eISSN: 1684-5315