Study of simple sequence repeat (SSR) polymorphism for biotic stress resistance in elite rice variety JGL 1798

  • G Siva Kumar
  • K Aruna Kumari
  • Ch. V. Durga Rani
  • RM Sundaram
  • S Vanisree
  • Md Jamaloddin
  • G Swathi

Abstract

To provide ready to use markers for back ground selection in marker assisted breeding of rice, we used GPP 2 as donor parent for xa13, Xa21, Gm4 resistance to bacterial blight, gall midge and NLR 145 as another donor parent for Pi-kh gene resistance to blast and JGL 1798 as recurrent parent was investigated using 128 simple sequence repeat (SSR) primers covered on chromosome number 1-12. The results reveal that 36 HRM primers showed distinct polymorphism among the donor and recurrent parents studied indicating the robust nature of microsatellites in revealing polymorphism. Based on this study, the large range of similarity values for related cultivars using microsatellites provides greater confidence for the assessment of simple sequence repeats (SSR) polymorphism.

Keywords: Simple sequence repeats (SSR), parental polymorphism, JGL 1798, hyper variable markers

African Journal of Biotechnology Vol. 12(40), pp. 5833-5838

Author Biographies

G Siva Kumar
Institute of Biotechnology, College of Agriculture, ANGRAU, Rajendranagar, Hyderabad-30, India
K Aruna Kumari
Institute of Biotechnology, College of Agriculture, ANGRAU, Rajendranagar, Hyderabad-30, India
RM Sundaram
Directorate of rice research, Rajendranagar, Hyderabad-30, India.
S Vanisree
Agricultural Research Institute, Rice Section, Rajendranagar, Hyderabad-30, India..
Md Jamaloddin
Institute of Biotechnology, College of Agriculture, ANGRAU, Rajendranagar, Hyderabad-30, India
G Swathi
Institute of Biotechnology, College of Agriculture, ANGRAU, Rajendranagar, Hyderabad-30, India
Published
2016-06-03
Section
Articles

Journal Identifiers


eISSN: 1684-5315