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African Journal of Biotechnology

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An improved plating assay for determination of phage titer

Liu Yang, Chao Li, Shao-Qin Zhai, Hua Zheng, Li-Zhi Fu, Da-Jun Li

Abstract


Phage is a virus that is parasitic on bacteria. It is very important to determine the titer of test sample in the study of phage. In this study, an improved plating assay was developed for detection of the number of recombinant phage Cap-T7 present in a test solution at a certain dilution point by counting the plaque forming units. The data demonstrated that the improved plating assay is fast, useful, and convenient for the determination of the phage titer in a sample.

Keywords: Phage Cap-T7, detection method, plaque forming units




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