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African Journal of Biotechnology

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Response of planktonic bacteria of New Calabar River to zinc stress

C O Nweke, J C Okolo, C E Nwanyanwu, C S Alisi

Abstract


Toxicity of Zn2+ on four planktonic bacteria isolated from New Calabar River water was assessed via dehydrogenase assay. Pure cultures of the bacterial strains were exposed to various Zn2+  concentrations (0.2 - 2.0 mM) in a nutrient broth amended with glucose and TTC. The responses of the bacterial strains to Zn2+ is concentration-dependent. At 0.2 mM, Zn2+ stimulated dehydrogenase activity in Proteus sp. PLK2 and Micrococcus sp. PLK4. In all strains, dehydrogenase activity was progressively inhibited at concentrations greater than 0.2 mM. The IC50 ranges from 0.236 ± 0.044 to 0.864 ± 0.138 mM. Total inhibition occurred at concentrations ranging from 1.283 ± 0.068 to 2.469 ± 0.045 mM. The order of zinc tolerance is: Micrococcus sp. PLK4 > Proteus sp. PLK2 > Pseudomonas sp. PLK5 > Escherichia sp. PLK1. The result of the in vitro study indicated that the bacterial strains are sensitive to Zn2+ stress. Therefore, Zn2+ contamination would pose serious threat to their metabolism in natural environments.

Key words: New Calabar River, zinc toxicity, planktonic bacteria, dehydrogenase.




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