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African Journal of Biotechnology

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Genetic variation within and between three Vietnamese pine populations (Pinus merkusii) using random amplified polymorphic DNA (RAPD) markers

Ho Manh Tuong, Nguyen Thu Giang, Chu Hoang Ha, Le Van Son

Abstract


Pinus merkusii is an important species in Vietnam with many economic and biological contributions. The information on diversity within and between populations of a species is necessary for plantation programs, breeding and conservation strategies. Genetic diversity of three Vietnamese populations (NA, QB and QN) was analyzed using the random amplified polymorphic DNA (RAPD) markers. Nine RAPD primers produced 82 markers, 77 of which were polymorphic with 93.9% of polymorphism. The results showed higher genetic variation within populations (72%) than between populations (28%) and low Nei’s genetic differentiation index among populations (0.1867). The populations also clustered based on PCoA analysis where cluster I included NA and QB populations and Cluster II, the QN population. These results suggest that P. merkusii populations in Vietnam is necessary to develop the genetic resources.

Keywords: DNA markers, genetic diversity, Pinus merkusii, random amplified polymorphic DNA (RAPD), Vietnam




http://dx.doi.org/10.5897/AJB2016.15309
AJOL African Journals Online