Optimization, purification and characterization of recombinant L-asparaginase II in Escherichia coli

  • Trang Thi Hien Nguyen
  • Cuong Tien Nguyen
  • Thanh Sy Le Nguyen
  • Tuyen Thi Do
Keywords: Characterization, Erwinia chrysanthemi, L-asparaginase, purification, tumor cell line.

Abstract

We studied optimal L-asparaginase sequence from GenBank accession number X12746 encoding for Lasparaginase from Erwinia chrysanthemi NCPPB1125. The expression level of recombinant Lasparaginase was determined as 78% of the total proteins. The purified L-asparaginase had a molecular mass of 37 kDa with specific activity of 312.8 U/mg. Kinetic parameters, Km, Vmax, Kcat and Kcat/Km of purified enzyme were found to be 0.5 mM, 500 U/mg, 14.9  103 s-1, and 29.9 X 103 mM-1s-1, respectively. Temperature and pH optimum were observed at 45ºC and pH 7.5, respectively. The enzyme exhibited about 20 and 60% retention of activity following 100 min incubation at 55 or 40°C, respectively. The activity of enzyme was inhibited by EDTA, Hg2+, Cu2+, Ni2+, and enhanced by Mg2+. Detergents (Tween 20, Tween 80, Triton X-100, and Triton X-114) decreased enzyme activity. DTT and DMSO at appropriate concentrations enhanced enzyme activity. In vitro anti-cancer activity was performed using different tumor cell lines. Concentration of recombinant L-asparaginase at 50 μg/ml inhibited 45.32, 48.22, 53.68, 51.22% with HL-60, P388, P3X63Ag8, SP2/0-Ag14 cell lines.  Recombinant L-asparaginase was expressed successfully in Escherichia coli with high expression level, had a high specific activity and antiproliferative effect on several tumor cell lines.


Key words: Characterization, Erwinia chrysanthemi, L-asparaginase, purification, tumor cell line.

Published
2016-08-16
Section
Articles

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eISSN: 1684-5315