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African Journal of Biotechnology

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Enzyme activity of a Phanerochaete chrysosporium cellobiohydrolase (CBHI.1) expressed as a heterologous protein from Escherichia coli

RL Howard, P Masoko, E Abotsi

Abstract


The aim of this study was to produce a secreted, heterologously expressed Phanerochaete chrysosporium cellobiohydrolase (CBHI.1) protein that required no in vitro chemical refolding and to investigate the cellulolytic activity of the clone expressing the glutathione S-transferase (GST) fused CBHI.1 protein. Plate enzyme activity screening of E. coli cells transformed with pGEXcbhI.1 vector on carboxy-methyl-cellulose (CMC) produced several clones which produced clearing zones on CMC when induced. A randomly selected representative pGEXcbhI.1 clone produced hydrolysis on both Avicel and CMC when induced. Crude protein extracts obtained from the induced pGEXcbhI.1 clone exhibited time dependent enzymatic activity against both CMC and Avicel.



Key words: Phanerochaete chrysosporium, cellobiohydrolase, cellulase activity, heterologous expression.


African Journal of Biotechnology Vol.2(9) 2003: 296-300



AJOL African Journals Online