Comparative biochemical and molecular evaluation of swarming of Proteus and effects of anti-swarm agents

  • BA Iwalokun Biochemistry Department, Lagos state University, P.M.B. 1087, Apapa, Lagos, Nigeria
  • YA Olukosi Genetics Division, Nigerian Institute of Medical Research (NIMR), 6 Edmond Crescent, Yaba – Lagos, P.M.B. 2013, Lagos, Nigeria
  • A Adejoro Biochemistry Department, Lagos state University, P.M.B. 1087, Apapa, Lagos, Nigeria
  • JA Olaye Biochemistry Department, Lagos state University, P.M.B. 1087, Apapa, Lagos, Nigeria
  • O Fashade Biochemistry Department, Lagos state University, P.M.B. 1087, Apapa, Lagos, Nigeria
Keywords: <I>Proteus</I>, swarming, urea, SDS, Tris

Abstract

In addition to inadequate understanding of swarming motility and virulence of Proteus, there is paucity of information on the relative effectiveness of the various anti-swarm agents. The anti-swarming effects of urea, sodium dodecylsulphate (SDS) and trihydroxymethylglycine (Tris) on 40 clinical isolates of Proteus Spp. were comparatively investigated and plasmids associated with swarming were characterized. The three substances elicited a comparable concentration-dependent anti-swarming property at 0.25 – 1.25% on nutrient agar. Anti-swarm agents displayed heterogeneity in their ability to cause significant decreases in the expression of virulence factors. Swarm motility was further found to be strongly associated with the expression of virulence factors in these strains. Of the Proteus strains tested, 32 were found to harbour 1 – 4 plasmids of size ranging from 6.0 – 33.5 kb. Plasmid curing resulted in loss of swarming in 65.6% of these strains. In order to reduce the risk of infection with virulent Proteus strains, the laboratory use of urea and SDS is suggested.

Key Words: Proteus, swarming, urea, SDS, Tris.

African Journal of Biotechnology Vol.3(1) 2004: 99-104

Author Biography

BA Iwalokun, Biochemistry Department, Lagos state University, P.M.B. 1087, Apapa, Lagos, Nigeria
Phone: 234-1-2601089
Published
2004-06-29
Section
Articles

Journal Identifiers


eISSN: 1684-5315