Biolistic transformation of Saccharomyces cerevisiae with β-glucosidase gene from Cellulomonas biazotea

  • S Parvez National Institute for Biotechnology & Genetic Engineering, P.O. Box 577, Jhang Road, Faisalabad, Pakistan
  • Z Mukhtar National Institute for Biotechnology & Genetic Engineering, P.O. Box 577, Jhang Road, Faisalabad, Pakistan
  • F Rashid National Institute for Biotechnology & Genetic Engineering, P.O. Box 577, Jhang Road, Faisalabad, Pakistan
  • MI Rajoka National Institute for Biotechnology & Genetic Engineering, P.O. Box 577, Jhang Road, Faisalabad, Pakistan
Keywords: Biolistic transformation, &beta, -glucosidase, <I>Cellulomonas biazotea, Saccharomyces cerevisiae</I>

Abstract

A β-glucosidase genomic DNA from Cellulomonas biazotea NIAB 442 was isolated and coated onto tungsten microprojectiles for direct transformation of the gene into Saccharomyces cerevisiae. Transformation of β-glucosidase into S. cerevisae conferred the ability to hydrolyse esculin and cellobiose, indicated that the gene is expressed in the bombarded yeast.

Key Words: Biolistic transformation, β-glucosidase, Cellulomonas biazotea, Saccharomyces cerevisiae.

African Journal of Biotechnology Vol.3(1) 2004: 112-115
Published
2004-06-29
Section
Articles

Journal Identifiers


eISSN: 1684-5315