Biolistic transformation of Saccharomyces cerevisiae with β-glucosidase gene from Cellulomonas biazotea
AbstractA β-glucosidase genomic DNA from Cellulomonas biazotea NIAB 442 was isolated and coated onto tungsten microprojectiles for direct transformation of the gene into Saccharomyces cerevisiae. Transformation of β-glucosidase into S. cerevisae conferred the ability to hydrolyse esculin and cellobiose, indicated that the gene is expressed in the bombarded yeast.
Key Words: Biolistic transformation, β-glucosidase, Cellulomonas biazotea, Saccharomyces cerevisiae.
African Journal of Biotechnology Vol.3(1) 2004: 112-115