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Biolistic transformation of Saccharomyces cerevisiae with &beta;-glucosidase gene from <i>Cellulomonas biazotea</i>


S Parvez
Z Mukhtar
F Rashid
MI Rajoka

Abstract

A β-glucosidase genomic DNA from Cellulomonas biazotea NIAB 442 was isolated and coated onto tungsten microprojectiles for direct transformation of the gene into Saccharomyces cerevisiae. Transformation of β-glucosidase into S. cerevisae conferred the ability to hydrolyse esculin and cellobiose, indicated that the gene is expressed in the bombarded yeast.


Key Words: Biolistic transformation, β-glucosidase, Cellulomonas biazotea, Saccharomyces cerevisiae.


African Journal of Biotechnology Vol.3(1) 2004: 112-115