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African Journal of Biotechnology

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Analysis of genetic diversity in bambara groundnut [Vigna subterranea (L.) Verdc] landraces using amplified fragment length polymorphism (AFLP) markers

Wazael H Ntundu, Inga C Bach, Jørgen L Christiansen, Sven B Andersen

Abstract


Amplified fragment length polymorphism (AFLP) was used to assess genetic diversity among 100 selected bambara groundnut [Vigna subterranea (L.) Verdc] landraces from a diverse geographic area of Tanzania. Eleven informative AFLP primer combinations generated a total of 49 scorable polymorphic amplification fragments across the bambara groundnut accessions. Genetic distances between all accessions based on Jaccard's variability index ranged from 0.1 to 0.68, with a total average of 0.3. The results showed that bambara groundnut landraces from Tanzania form a genetically diverse population, and AFLP markers can be effectively employed to assess genetic diversity and to measure genetic relationship among accessions. Cluster analysis revealed that bambara groundnut from Tanzania constitute two major groups in line with their putative geographic origins, one genetically distinct group from the Southern agro-ecological zone and a mixed group with accessions from Central, Lake Victoria and Western agro-ecological zones. The clustering of accessions compared relatively well to clustering based on phenotypic characters. However, correlation of the AFLP marker distances with phenotypic distances showed r-values of 0.4 only.

Key Words: AFLP markers; genetic diversity; landraces; Tanzania; Vigna subterranean.

African Journal of Biotechnology Vol. 3 (4), 2004: 220-225



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