African Journal of Biotechnology

Log in or Register to get access to full text downloads.

Remember me or Register

Studies on improved agrobacterium - mediated transformation in two indica rice (Oryza Sativa L.)

Vinod Saharan, Ram C Yadav, Neelam R Yadav, Khushi Ram


Agrobacterium tumefaciens strain EHA 105 carrying binary vector pCAMBIA 1301 was used for transformation in two economically important highly recalcitrant indica rice cultivars HKR-46 and HKR-126. High concentrations of acetosyringone in the agrobacterium culture and co-cultivation medium proved to be indispensable for successful transformation. Embryogenic scutellar calli were used for transformation studies. Binary vector pCAMBIA 1301 have been proved efficient for transformation. The percent transient GUS expression found to be higher in cutivar HKR-126 (44.4%) as compared to HKR-46 (28.9%). The percent recovery of hygromycin resistant calli after 4-6 weeks on selection medium was maximum in HKR-126 (52.6%).
Key Words: Agrobacterium, indica rice, transformation, acetosyringone.
African Journal of Biotechnology Vol.3(11) 2004: 572-575

AJOL African Journals Online