Studies on improved agrobacterium - mediated transformation in two indica rice (Oryza Sativa L.)

  • Vinod Saharan The Institutes for Applied Research, Ben-Gurion University, P.O.B. 653, Beer-Sheva 84105, Israel.
  • Ram C Yadav Department of Biotechnology and Molecular Biology, CCS HAU, Hisar 125004 (Haryana), India.
  • Neelam R Yadav Department of Biotechnology and Molecular Biology, CCS HAU, Hisar 125004 (Haryana), India.
  • Khushi Ram Rice Research Station, Kaul, (Haryana), India
Keywords: Agrobacterium, indica rice, transformation, acetosyringone

Abstract

Agrobacterium tumefaciens strain EHA 105 carrying binary vector pCAMBIA 1301 was used for transformation in two economically important highly recalcitrant indica rice cultivars HKR-46 and HKR-126. High concentrations of acetosyringone in the agrobacterium culture and co-cultivation medium proved to be indispensable for successful transformation. Embryogenic scutellar calli were used for transformation studies. Binary vector pCAMBIA 1301 have been proved efficient for transformation. The percent transient GUS expression found to be higher in cutivar HKR-126 (44.4%) as compared to HKR-46 (28.9%). The percent recovery of hygromycin resistant calli after 4-6 weeks on selection medium was maximum in HKR-126 (52.6%).
Key Words: Agrobacterium, indica rice, transformation, acetosyringone.
African Journal of Biotechnology Vol.3(11) 2004: 572-575
Published
2005-03-23
Section
Articles

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eISSN: 1684-5315