Purification and characterization of a protease from Thermophilic bacillus strain HS08

  • H Guangrong
  • Y Tiejing
  • H Po
  • J Jiaxing
Keywords: Neutral protease, purification, characterization, Thermophilic bacillus, thermophilic protease, serine protease.


The purification and characterization of a thermophilic neutral protease from Thermophilic bacillus strain HS08, originally isolated from a soil sample collected from the Tulufan Crater of China, is presented in this paper. The purification steps included ammonium sulfate precipitation, with columns of DEAE-Sepharose anion exchange chromatography and Sephacryl S-100HR on AKTA purifier 100 protein liquid chromatography. The method gave a 4.25 fold increase of the specific activity and had a yield of 5.1%. The molecular weight of the protease was found to be around 30.9 kDa by SDS-PAGE technique. The optimal pH and optimal temperature of the protease were at pH 7.5 and 65oC,
respectively. The protease was found stable during the 1 h incubation at 50°C. The protease activity showed wide range of variation in the presence of different reagents: it was inhibited remarkably by
EDTA or PMSF and was almost activated by 2 mM Zn2+, even though it was only marginally inhibited by other inhibitors. We concluded that the protease was a Zn2+-acitived serine protease. Substrates
specificity tests indicated that azocasein was the best substrate among the three substrates tested (azocasein, casein, and BSA).

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eISSN: 1684-5315