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with Agrobacterium tumefaciens (GV3101) that contained the binary vector plasmid pADI. Transformed shoots were obtained on Murashige and Skoog medium supplemented with 1.50 mgl-1 IAA, 0.10mgl-1
BAP, 0.01 mgl-1 NAA and 6 mgl-1 kinetin. Transformants were selected by using only 100 mgl-1 of kanamycin and 4 days of pre-culture. Putative transformants were confirmed for transgene insertion through polymerase chain reaction (PCR) analysis. From the inoculated explants, 6.66% produced transgenic shoots.