Integration of C4-specific ppdk gene of Echinochloa to C3 upland rice and its photosynthesis characteristics analysis
Abstract
Pyruvate orthophosphate dikinase (PPDK) plays a key role in C4 photosynthetic pathway. The enzymatic reaction is one of the rate-limiting steps of the C4 photosynthetic pathway. In this paper, the gene encoding Echinochloa pyruvate orthosphate dikinase (GenBank accession number: AB289641) was introduced into H65, a upland rice variety, using an Agrobacterium-mediated system. Southern
hybridization, Northern hybridization and enzyme activity determination all confirmed the effective expression of Echinochloa C4 type PPDK in upland rice with the enzyme activity being elevated 1 - 11 folds. However, no appreciable change demonstrated in carbon assimilation of the transgenic upland rice though increased photoinhibition was noted under high light intensity.
hybridization, Northern hybridization and enzyme activity determination all confirmed the effective expression of Echinochloa C4 type PPDK in upland rice with the enzyme activity being elevated 1 - 11 folds. However, no appreciable change demonstrated in carbon assimilation of the transgenic upland rice though increased photoinhibition was noted under high light intensity.
