African Journal of Biotechnology

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The effects of drying on the chemical components of essential oils of Calendula officinalis L.

OO Okoh, AP Sadimenko, OT Asekun, AJ Afolayan


Calendula officinalis is a medicinal plant whose essential oils are used for various purposes. The oils were extracted by hydrodistillation from fresh leaves, dry leaves and fresh flowers of the herb yielding
0.06, 0.03 and 0.09%, respectively. The analysis of the oils by GC-MS revealed a total of 30, 21 and 24 compounds from the fresh leaves, dry leaves and the flowers in the same order. Sesquiterpenoids
dominated the fresh leaves (59.5%) and flowers (26%), while the monoterpenes dominated the oil in the dry leaves (70.3%). T-muurolol (40.9%) predominated in the fresh leaf oil; a-thujene (19.2%) and dcadinene (11.8%) were also present in high quantities. Whereas, 1,8-cineole (29.4%), g-terpenene (11.6%), d-cadinene (9.0%), b-pinene (6.9%) and a-thujene (6.3%) were the major components in the dry leaf oil. In the fresh flower oil, a-thujene (15.9%), d-cadinene (13.1%) and d-cadinene (10.9%) were the major components. The significance of the effect of drying on essential oil composition of this plant is discussed.

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