Protoplasting impact on polyketide activity and characterization of the interspecific fusants from Streptomyces
  • H Lazim
  • N Slama
  • M Abbassi
  • I Barkallah
  • AB Hassen
  • F Limam
Keywords: Streptomyces, polyketide antibiotic, gene transfer, protoplast regeneration, fusion.

Abstract

The influence of protoplasting and protoplast regeneration on antibiotic activity, transfer of biosynthesis encoding genes in local Streptomyces spp. CN207 was studied. The frequency of regenerated protoplasts in the lag phase was 1.7x103 CFU/ml, in the beginning of the exponential phase 0.4x102 CFU/ml, in the exponential growth phase 2.5x103 CFU/ml, and 1.0x105 CFU/ml in stationary phase. The protoplast formation and regeneration technique resulted in a new isolate strain of
Streptomyces spp.PR01 that produced approximately 5 fold more Streptomyces spp. CN207 antibiotic. The protoplast fusion resulted in increased isolation of variants with higher antibiotic activity.
Recombinant Streptomyces coelicolor PF04 was increased 10 times more than the wild strain. Theprocesses also affected on the strain resistance to some antibiotics but had no effect on the components of the antibiotic. The characteristics of this recombinant product were similar considerably to Streptomyces spp. CN207 product. Our data, in principal, indicate the possibility of transferring antibiotics cluster genes by fusion and provide a starting point for genetic and biochemical
investigations of CN207 biosynthesis.
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eISSN: 1684-5315