Effects of plant growth regulators on callus induction from Cananga odorata flower petal explant

  • Z Nurazah
  • M Radzali
  • A Syahida
  • M Maziah
Keywords: Cananga odorata, 1-naphtalene acetic acid, 6-benzylaminopurine, callus culture.


The Cananga odorata callus was initiated from petals of the C. odorata flowers on MS medium and B5 vitamins containing 30 g/L sugar and 3 g/L agar. The medium was also supplemented with different concentrations of 1-naphtalene acetic acid (NAA), and combinations of NAA with 6-benzylaminpurine (BAP) as the plant growth regulators. It was observed that the C. odorata callus could be induced on media containing the combination of 3 mg/L NAA and 0.5 mg/L BAP. High concentration of NAA gave rise to pale, whitish and friable callus after 1 - 2 weeks of culture. The optimum pH for the cell culture was about 5.7 and incubation at 25 ± 2°C, in totally dark condition.

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eISSN: 1684-5315