Biodegradation of biodiesel/diesel blends by Candida viswanathii

  • JS Junior
  • AP Mariano
  • D de Angelis
Keywords: Diesel, biodiesel, blends, biodegradation, bioaugmentation, TBHQ.


This work is aimed to assess the aerobic biodegradation of biodiesel/diesel blends (0, 2, 5, 20 and 100%, v/v) by Candida viswanathii. The biodegradation potential of the inoculum was assessed with the redox indicator 2,6-dichlorophenol indophenol (DCPIP) test and with  respirometric experiment in biometer flasks (250 mL) used to measure the microbial CO2 production. In the latter, the inoculum was added to a contaminated soil with the blends (addition of 50 mL of fuel/Kg of soil from a noncontaminated site). C. viswanathii was able to increase significantly (approximately 50% in terms of CO2 production) the biodegradation in soil of biodiesel/diesel blends and neat biodiesel since it preferable biodegrades biodiesel. Without inoculum the biodegradation of diesel oil was higher than biodiesel and blends (47.3, 51.1, 5.7 and 22.1% in terms of CO2 production by B2, B5, B20 and B100, respectively) presumably due to the presence of the antioxidant terc-butyl-hydroquinone (TBHQ) in the biodiesel

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eISSN: 1684-5315