Characterization of a new synthetic wheat – Aegilops biuncialis partial amphiploid

  • F Tan
  • J Zhou
  • Z Yang
  • Y Zhang
  • L Pan
  • Z Ren
Keywords: SDS-PAGE, A-PAGE, fluorescence in situ hybridization, wheat – Aegilops biuncialis partial amphiploid.

Abstract

The aim of the experiments was to identify the synthetic wheat – Aegilops biuncialis germplasm Line 15-3-2 with 42 chromosomes. Morphologically, the spike of line 15-3-2 is intermediate to those of its wheat and Aegilops parents. Line 15-3-2 displays stable fertility and immunity to wheat powdery mildew and stripe rust. The seed storage protein electrophoresis showed that Line 15-3-2 presented almost all gliadin bands and a majority of glutenin subunits of its parents. Fluorescence in situ hybridization
(FISH) suggested that Line 15-3-2 contained all 14 D-genome chromosomes and chromosome 5U. Therefore, it could be concluded that Line 15-3-2 is a new synthetic wheat – A. biuncialis partial amphiploid, and could be used to transfer the disease resistance genes to wheat.
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eISSN: 1684-5315