Molecular diagnostics and phylogenetic analysis of ‘Candidatus phytoplasma asteris’ (16SrI- Aster yellow group) infecting banana (Musa spp.)

  • BR Singh
  • A Aminuddin
  • AA Al-Khedhairy
  • F Al-Qurainy
  • J Musarrat
Keywords: Phytoplasma, polymerase chain reaction, cloning, 16SrDNA, banana, phylogenetic analysis

Abstract

The presence of phytoplasma in diseased banana plants exhibiting acute stunting symptoms was detected by the polymerase chain reaction using a primer set specific for the 16SrRNA gene of phytoplasma. The amplified DNA fragments of 1500 bp were cloned in pGEMT-Easy plasmid cloning
vector and sequenced. The BLASTN and phylogenetic analyses revealed the infecting agents to be the closely related members of the ‘Candidatus phytoplasma asteris’ (16Sr I-Aster yellow) group.
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eISSN: 1684-5315