Validated high performance liquid chromatographic (HPLC) method for analysis of zerumbone in plasma

  • EEM Eid
  • AB Abdul
  • AS Al-Zubairi
  • MA Sukari
  • R Abdullah
Keywords: ZER, humulene, HPLC, human plasma


Zerumbone (ZER) is a sesquiterpene derived from Zingiber zerumbet smith, family Zingiberaceae. It has been shown to possess anti-cancer and apoptosis-inducing properties against various human tumour cells as well as in vivo against a number of induced malignancies in mice. In this study a simple, specific and accurate high performance liquid chromatographic method for determination of ZER in micro-volumes human plasma (| 1.5 ml) was developed and validated. ZER and its analogue -Humulene
as internal standard were easy recovered by simple one step plasma protein precipitation using acetonitrile and separated in isocratic mobile phase, on reverse phase-C18 column. The effluent was monitored by Photodiode Array (PDA) detector and at a flow rate of 1.0 ml/min. The linearity of proposed method was 2 – 15 ìg/ml. The intra-day and inter-day coefficient of variation and percent error values of the method were less than 15% and mean recovery was more than 90% for both ZER and -
Humulene. This method was found to be precise, specific, accurate and robust for detection and analysis of ZER in human plasma.

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eISSN: 1684-5315