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African Journal of Biotechnology

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The use of cold shock in inducing triploidy in African mud catfish (Clarias gariepinus)

AM Hammed, HA Fashina-Bombata, AO Osinaike

Abstract


study was conducted to induce triploidy in African mud catfish Clarias gariepinus using cold shock. The fertilized eggs were exposed at one temperature regime of 0°C with varied shock treatments of 0, 15, 25 and 30 min. Some 3 min after fertilization, the success of triploidy was determined by the presence or absence of bent trunk in the fry as reported by (Aluko et al., 1997). The shock duration of 25 min at 0°C gave the best result of 55% hatch with survival at the end of the exposure regime of 43.5%. Shock exposure of 30 min gave 30% hatch while 15 and 20 min exposure were poor at 15% hatch.



http://dx.doi.org/10.5897/AJB10.265
AJOL African Journals Online