Differential expression of ozone-induced gene during exposures to salt stress in Polygonum sibiricum Laxm leaves, stem and underground stem

  • Shou-Hai Na
  • Chun-Pu Qu
  • Guan-Jun Liu
  • Chun Liu
  • Rui-Xuan Gu
  • Zhi-Gang Wei
  • Gui-Feng Liu
  • Chuan-Ping Yang

Abstract

The response of plants to environmental stresses is characterized by a number of physiological and biochemical changes that ultimately result from the selective increase or decrease in the biosynthesis of a large number of distinct proteins. In this report, we describe the characterization of an ozoneinduced transcript, PcOZI-1, which has been identified from Polygonum sibiricum Laxm by the rapid amplification of cDNA ends method (RACE). PcOZI-1 mRNA in untreated plants was detected at low levels in underground stem, leaves and at higher levels in stem. PcOZI-1 mRNA accumulation was transiently induced in stem 7-fold within the first 8 h of 3% NaHCO3 treatment. PcOZI-1 mRNA accumulation was also induced 22-fold in underground stem after 72 h. Sequence analysis of PcOZI-1 revealed that it encodes a 8.45 kDa basic protein that contains a putative signal peptide. Our results suggest that PcOZI-1 represents a novel stress-related protein that accumulates in response to the production of active oxygen species

.Keywords: Polygonum sibiricum Laxm, PcOZI-1, rapid amplification of cDNA ends, gene expression

African Journal of Biotechnology Vol. 9(33), pp. 5338-5342, 16 August, 2010

Author Biographies

Shou-Hai Na
College of Wildlife Sciences, Northeast Forestry University, Harbin 150040, P. R. China
Chun-Pu Qu
The Laboratory of Forest Genetics and Breeding and Biotechnology of Ministry of Education, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China
Guan-Jun Liu
The Laboratory of Forest Genetics and Breeding and Biotechnology of Ministry of Education, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China
Chun Liu
The Laboratory of Forest Genetics and Breeding and Biotechnology of Ministry of Education, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China
Rui-Xuan Gu
The Laboratory of Forest Genetics and Breeding and Biotechnology of Ministry of Education, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China
Zhi-Gang Wei
The Laboratory of Forest Genetics and Breeding and Biotechnology of Ministry of Education, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China
Gui-Feng Liu
The Laboratory of Forest Genetics and Breeding and Biotechnology of Ministry of Education, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China
Chuan-Ping Yang
The Laboratory of Forest Genetics and Breeding and Biotechnology of Ministry of Education, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China
Published
2013-08-09
Section
Articles

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eISSN: 1684-5315