Chemical constituents and antimicrobial activity of Goniothalamus macrophyllus (Annonaceae) from Pasoh Forest Reserve, Malaysia

  • AG Siti Humeirah
  • MA Nor Azah
  • M Mastura
  • J Mailina
  • JA Saiful
  • H Muhajir
  • AM Puad

Abstract

The essential oils from the twig and root of Goniothalamus macrophyllus were obtained by hydrodistillation and subjected to Gas Chromatography (GC-FID) and Gas Chromatography/Mass Spectrometry (GC-MS) using CBP-5 capillary column in order to determine their chemical composition. Both twig and root oils and four reference standard compounds (-pinene, linalool, geraniol and geranyl acetate) were evaluated for their antimicrobial properties against gram positive and gram negative bacteria, yeast and dermatophyte fungi using broth microdilution methods. The GCMS analysis revealed twenty-one and fourteen compounds from twig and root oils which represented 90.0 and 42.5% of the total oils, respectively. The oils were found to possess the following major components: twig: geranyl acetate (45.5%), geraniol (17.0%), linalool (12.7%) and camphene (7.5%); root: cyperene (9.8%), geranyl acetate (9.4%), geraniol (3.4%) and linalool (2.6%). Other components present in appreciable amounts in both essential oils were -pinene (0.8%) and benzaldehyde (0.5%). The root oil exhibited the most notable inhibitory activity (0.3 mg/ml) against Vancomycin intermediate-resistance Staphylococcus aureus (VISA 24), Staphylococcus epidermidis and Candida albicans. -pinene meanwhile inhibited the bacteria and fungal growth at 0.3 and 2.5 mg/ml. With regards to antimicrobial potential, -pinene superceeds linalool, geraniol and geranyl acetate, respectively.

Keywords: Goniothalamus macrophyllus, Annonaceae, essential oils, geranyl acetate, cyperene, geraniol, linalool, -pinene, antimicrobial activity

African Journal of Biotechnology Vol. 9(34), pp. 5511-5515, 23 August, 2010

Author Biographies

AG Siti Humeirah
Product Development and Formulation Programme, Medicinal Plants Division, Forest Research Institute Malaysia (FRIM) 52109 Kepong, Kuala Lumpur, Malaysia; Institute of Bioscience, University Putra Malaysia (UPM), 43400 Serdang, Selangor Darul Ehsan, Malaysia
MA Nor Azah
Product Development and Formulation Programme, Medicinal Plants Division, Forest Research Institute Malaysia (FRIM) 52109 Kepong, Kuala Lumpur, Malaysia
M Mastura
Bioactivity Programme, Medicinal Plants Division, Forest Research Institute Malaysia (FRIM) 52109 Kepong, Selangor, Malaysi
J Mailina
Product Development and Formulation Programme, Medicinal Plants Division, Forest Research Institute Malaysia (FRIM) 52109 Kepong, Kuala Lumpur, Malaysia
JA Saiful
Bioactivity Programme, Medicinal Plants Division, Forest Research Institute Malaysia (FRIM) 52109 Kepong, Selangor, Malaysi
H Muhajir
Institute of Bioscience, University Putra Malaysia (UPM), 43400 Serdang, Selangor Darul Ehsan, Malaysia; Department of Microbiology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor Darul Ehsan, Malaysia
AM Puad
Institute of Bioscience, University Putra Malaysia (UPM), 43400 Serdang, Selangor Darul Ehsan, Malaysia; Department of Animal Cell, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor Darul Ehsan, Malaysia
Published
2013-08-10
Section
Articles

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eISSN: 1684-5315