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African Journal of Biotechnology

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In vitro propagation and whole plant regeneration from callus in Datura (Datura stramonium. L)

S Amiri, SK Kazemitabar, GA Ranjbar, M Azadbakht

Abstract


Leaf explants and mature embryos of Datura were separately cultured in MS basal medium supplemented with 30 g/l sucrose, 8 g/l agar and different combination of 2,4,D (at three levels, 0, 1 and 2 mg/l) and kinetin (0, 0.25, 0.5 and 1 mg/l). The formed calli were transferred to regeneration media supplemented with BAP alone (at three levels, 1, 2 and 3 mg/l) or in combination with NAA (at four levels, 0, 0.02, 0.2 and 1 mg/l). The regenerated shoots were rooted in media containing IBA at three levels (0.5, 1 and 1.5 mg/l). The media containing 2 mg/l 2,4-D + 0.5 mg/l kinetin and 2 mg/l of 2,4-D alone were found to be the best treatments for callus induction from leaf and embryo explants, respectively. Moreover, the media containing 3 mg/l BAP + 1 mg/l NAA and 2 mg/l BAP + 1 mg/l NAA were found to be the best hormonal treatments to shoot regeneration from calli of leaf and embryo explants, respectively.
Also 0.5 mg/l IBA was found to be the best treatment for rooting of regenerated shoots.

Key words: Datura, leaf explants, embryo explants, calli.




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