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African Journal of Biotechnology

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Detection of genetically modified maize (Zea mays L.) in seed samples from Nepal

Hari Kumar Shrestha, Kae-Kang Hwu, Men-Chi Chang

Abstract


Maize is the second major cereal in Nepal; its food biosafety and ecological conservation is an important concern. To address this issue, it is necessary to detect genetically modified (GM) maize and establish a monitoring and regulatory system in Nepal. Currently, Nepal does not have legal regulations or labeling directives for GM maize. Therefore, the authors aimed to survey the current status of GM maize seeds in Nepal. First, they performed multiplex polymerase chain reaction (mPCR) to detect 8 GM maize lines in 46 maize seed samples from different locations in Nepal. Suspected samples were then verified by real-time PCR (RT-PCR) and screen-specific PCR. Based on current evidence, they can not identify any GM maize in the seed samples. This first report may formulate and implement a baseline for quality regulation and biodiversity conservation of maize seeds in Nepal.

Keywords: Genetically modified crops, maize, seeds, Nepal, multiplex polymerase chain reaction, real-time PCR

African Journal of Biotechnology Vol. 9(34), pp. 5581-5589, 23 August, 2010



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