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African Journal of Biotechnology

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De novo characterization of Lycoris sprengeri transcriptome using Illumina GA II

L Chang, J Chen, Y Xiao, Y Xia

Abstract


The Illumina GA II high-throughput sequencing technology was used to establish a transcriptome library from the bulbs of Lycoris sprengeri. A total of 24 million short read with an average length of 90 nt were generated, which produced 98,150 unigenes with a mean length of 385 nt. A total of 45.9% of these unigenes (45,052) were annotated using BLAST (E-value 1.0E-5) against the nr, SwissProt, Kyoto encyclopedia of genes and genomes (KEGG) and Clusters of orthologous groups (COG) databases. The unigenes were annotated with gene ontology (GO) and COG. Of these, 2,173 unigenes were found to be related to carbohydrate metabolism, and 544 were involved in starch and sucrose metabolism.

Key words: Lycoris sprengeri, Illumina GA II, transcriptome, carbohydrate metabolism.




AJOL African Journals Online